A centrifuge is a machine that uses centrifugal force to separate liquid and solid particles or components of a mixture of liquid and liquid. The centrifuge is mainly used to separate the solid particles in the suspension from the liquid, or to separate the two liquids of different density and incompatible liquid in the emulsion, such as separating the cream from the milk. Below the Hundred Bios Mall will introduce you to the practical operation technology of the laboratory centrifuge.
1 Centrifuge operation steps
(1) Power on: Turn on the power, turn on the power switch of the instrument, and self-test the centrifuge.
(2) Press the “Open†button, open the cover, unscrew the rotor cover, balance into the centrifuge tube, tighten the rotor cover, close the cover, and the indicator light of the “Open†button lights up. (If the cover is open after the power is turned on, this step is not required).
(3) Centrifugal condition setting: Set the temperature, speed and time of centrifugation by pressing the up and down arrows of the “Tempâ€, “Speed†and “Time†buttons. The centrifugal speed can be divided into two speeds per minute (rpm) and centrifugal force (cfg), which can be converted by pressing the up and down keys of the “Speed†button at the same time.
(4) Operation: After the centrifugal condition is set, press the “Start/Stop†button, the centrifuge will start, and the indicator light of the “Open†button will be off. When the set time is reached, the centrifuge automatically stops and a beep sounds. The indicator light of the “Open†button lights up. Press the “Open†button to open the cover, unscrew the rotor cover, and take out the centrifuge tube.
(5) If the sample is briefly centrifuged, simply press the “Short†button for a few seconds.
(6) If you need to stop centrifugation during centrifugation, you need to press the “Start/Stop†button again.
(7) Shutdown: After centrifugation, wipe off the condensate from the centrifuge chamber with a dry soft cloth, turn off the centrifuge power, and record the centrifuge usage.
2 Centrifuge maintenance (1) Correct installation of the centrifuge Power supply voltage: Voltage fluctuations should meet the national standard of +10%, otherwise some manufacturers' centrifuges will not operate normally. If the voltage fluctuates above this, it is recommended to use a voltage regulator to comply with this grid.
The ground wire should be strong: the wiring of the power supply of the room should meet the requirements. China is a three-phase four-wire system. The three-phase is the three-phase in industrial power. When three-phase electricity is used, the neutral line is separated from the ground line. More importantly, whether it is three-phase industrial power or not, the general single-phase power is good, and the ground line should be reliable to avoid leakage. The ground wire cannot be connected to the heating pipe or the water pipe.
The ground should be flat and firm: for large centrifuges, the floor should be firm at the installation site to ensure that the rotor of the centrifuge is working properly and not recessed. The ground at the installation site should be flat and firm.
Find a good level: find a more accurate small level, open the centrifuge cover, find the level on the main shaft, and adjust the level of the adjustment bolts next to the 4 casters to find the level. Note that one of the feet cannot be lost.
(2) The centrifugal loader and the leveling centrifuge are designed and manufactured, and the imbalance caused by the machining error of the rotor has been remedied by the dynamic balance test. However, all centrifuges have their allowed samples. Unbalanced amount. Centrifuge manufacturers strive to give larger values ​​in order to compete with other manufacturers on the maximum unbalance limit allowed by the centrifuge. At this larger value, the centrifuge can be operated, but the imbalance force generated at this time suddenly hits the bearing and the bracket at n times per minute, and the centrifuge is damaged. Therefore, users should try to find a good balance and centrifuge for those expensive centrifuges, which is good for the life of the centrifuge.
(3) Cleaning up the water When the centrifuge is running, it uses refrigeration. Because of the moisture of the air, it is frosted in the centrifugal chamber, and it is frosted into water after shutdown. Most foreign low-speed large-capacity centrifuges have no drainage holes, and the water in the centrifugal chamber is more and more accumulated. At this point, the user should remove the rotor and clean up the accumulated water. When reinstalling the rotor, be sure to install it to avoid accidents.
(4) Corrosion of aluminum alloy The rotor of the centrifuge is generally made of aluminum alloy. When it is corroded, the strength is reduced and it is easy to cause an accident. The aluminum alloy is susceptible to liquid corrosion. After cleaning, it is blown dry with a hair dryer, or inverted for a period of time to confirm that it can be used. Some blood stations imported centrifuges, and there should be 6 plastic trays in 6 aluminum cups. The plastic tray should be placed in a blood bag to avoid direct placement in the aluminum cup. Note the test tube for cracks. If the test tube (especially the test tube used repeatedly) has cracks, it must not be used. Otherwise, the test tube rupture will cause the rotor to lose its balance during rotation, which may cause a vicious accident such as broken shaft.
(5) Broken shaft accident The rotor is not placed well, or the sample unbalance is too large, and the centrifuge starts. Modern imported centrifuges have unbalanced protection, that is, when the unbalance amount exceeds a certain limit, the power should be automatically cut off to stop the centrifuge. However, under the above circumstances, it is already late, and a serious accident will occur. One of the malignant accidents is the broken shaft. Due to the sudden break of the shaft during high-speed rotation, the centrifugal rotor has no support, and the centrifuge is turned into a chaotic collision, which can turn the centrifuge to a 270° angle. At this time, if there are people or equipment next to it, it will cause casualties and damage to the equipment.
Peru Squid ,Peru Jumbo Flying Squid,Peru jumbo flying squid
Zhoushan Boda Aquatic Products Co.,Ltd , https://www.baida-aquatic.com