A double-sword combination of tumor efficacy test tools - B-hCTLA4/hOX40 double immunological checkpoint humanized mice
Guide
As the application of tumor immunotherapy is in full swing, the combination of different antibody drugs has begun to attract attention. What is the efficacy of the combination of antibody and drug? In the process of inquiry, it is inevitable to use humanized mice with double immunological checkpoints. Today, we introduce a double-immunization checkpoint humanized mouse, B-hCTLA4/hOX40 mice.
Genetic background
Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), also known as CD152, plays an important regulatory role in the activation of T cells . CTLA4 is the first clinically focused immune target and plays an important role in the negative regulation of immune responses. The CTLA4 antibody is also the first antibody drug approved by the FDA for the treatment of advanced melanoma.
Figure 1. The role of CTLA4 and its antibodies in tumor immunotherapy [1]
OX40, also known as Tnfrsf4 (Tumor necrosis factor receptor superfamily, member 4), is expressed mainly on the surface of activated CD4 + and CD8 + T cells , and binding to OX40 ligand can stimulate the activation of CD8 + T cells. Through the co-activation effect of the OX40/OX40L signal, the functions of T cells, including the production and proliferation of cytokines and the survival of T cells, are further enhanced. OX40 antibody activator (Agonist) reduces T regs in tumors and increases antitumor activity.
Figure 2. OX40 promotes T cell proliferation [2]
Bio-Stuart uses the self-developed B-hCTLA4/hOX40 double-sourced mouse to provide an effective model and powerful tool for studying the in vivo efficacy of the target antibody drug!
Basic Information
Protein expression analysis 
Figure 3. Activation and flow cytometry of B-hCTLA4/hOX40 humanized mouse spleen cells
The results showed that mCTLA4 cells were detectable in C57BL/6 mice. In B-hCTLA4/hOX40 homozygous mice, hCTLA4 cells were detected.
Figure 4. Activation and flow cytometry of B-hCTLA4/hOX40 humanized mouse spleen cells
The results showed that mOX40 + cells were detectable in C57BL/6 mice . h OX40 + cells were detected in B-hCTLA4/hOX40 homozygous mice .
Reference materials:
1. Https://gss3.bdstatic.com/7Po3dSag_xI4khGkpoWK1HF6hhy/baike/c0%3Dbaike150%2C5%2C5%2C150%2C50/sign=3cf5552b865494ee932f074b4c9c8b9b/241f95cad1c8a786063c9e9a6d09c93d70cf5014.jpg
2.http://
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The company is currently located in Yizhuang, Beijing. It has branches in Shanghai Zhangjiang, Jiangsu Haimen and Boston, USA, and has offices in Wuhan, Guangzhou and Chengdu. Where.
Established in the United States in 2008 and successfully operated. In 2009, Beijing Baiao Saitu Headquarters was established, and in 2012, the headquarters was relocated to Beijing Yizhuang Economic and Technological Development Zone.
Bio-Saitu is a high-tech company based on a stable and efficient gene editing technology platform, with model animal customized services, important model animal development and large-scale breeding and supply, in vivo pharmacological efficacy evaluation services, and antibody drug research and development outsourcing services. Biopharmaceutical companies.
The company adheres to the principle of pursuing better human health and aims to provide high quality products and services for new drug research and development.
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The lamp panel is divided into ordinary infrared lamps (Dingyuan and Guanglei) and array lamps. The array camera is also divided into 1W, 2W and 3W. Therefore, when choosing infrared lamps, if it is an ordinary infrared lamp, try to choose Dingyuan and 3W array lamps. Since the appearance of infrared lamps is generally invisible, some cottage cameras are filled with Guanglei and 1W array cameras are filled with 3W array cameras. Therefore, try to choose a large brand when choosing cameras.
Maximum Aperture
The real value of the maximum aperture is to improve the light intake under low light conditions, so as to achieve the best exposure combination. In general, the ability to visualize the lens is not required for shooting landscapes. In addition, the lens caliber is not required for shooting landscapes except in particularly dark places. However, when the 70-200mm zoom lens is equipped with a 2-fold zoom lens to make the telephoto section become 400mm and AF is used for automatic focusing, it is better to choose a lens with the largest aperture of F2.8. Large aperture is conducive to accurate focusing in dark conditions. When shooting people with a large aperture lens, even in places where the light is weak, the handset can also use natural light to shoot. In addition, the lens with the largest aperture can bring fast shutter speed, so sports photography also needs a large aperture lens.
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focal length
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A 100mm macro lens is enough for traditional SLR to shoot flowers. The 100mm macro lens can be used for equal magnification photography, which can make the flowers very large. However, when taking equal magnification photography or near equal magnification photography, due to the depth of the long focus scene, it is easy to shake, so anti shake measures should be considered when taking photos. From this point of view, 50mm macro lens is easier to use on digital SLR.
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